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EFFECTS OF TRIBUTYLIN ON BIOMEMBRANES: ALTERATION OF FLOW CYTOMETRIC PARAMETERS AND INHIBITION OF NA+, K+-ATPASE TWO-DIMENSIONAL CRYSTALLIZATION
Citation:
Zucker, R. AND e. al. EFFECTS OF TRIBUTYLIN ON BIOMEMBRANES: ALTERATION OF FLOW CYTOMETRIC PARAMETERS AND INHIBITION OF NA+, K+-ATPASE TWO-DIMENSIONAL CRYSTALLIZATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/488 (NTIS PB89218192), 1990.
Description:
Carboxyfluorescein diacetate (ICFDA) is a lipophilic nonfluorescent molecule that readily crosses the cell membrane. In the cytoplasm, it is hydrolyzed by nonspecific esterases to carboxyfluorescein (CF), a negatively charged fluorescent molecule, which is retained incompletely by cells with an intact plasma membrane. Exposure (4hr) of the murine erythroleukemic cell (MELC) to micromolar quantities (0.1 to 5.0 KM) of tributylin (TBT) results in increased cellular CF fluoresence. The increase occurs within a range below a critical value of the product (CPV) of the concentration (C) of TBTxduration (T) of exposure to TBT. luorescence increase is a sensitive indicator of the interation of TBT with the cell: it is observed following exposure to 0.1 uM TBT for 4 hr at 37oC. n the range above the CPV, cellular CF fluorescence is reduced apparently resulting from perturbation of membrane structure.