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DETECTION OF HORIZONTAL GENE TRANSFER BY NATURAL TRANSFORMATION IN NATIVE AND INTRODUCED SPECIES OF BACTERIA IN MARINE AND SYNTHETIC SEDIMENTS
Citation:
Steward, G. AND C. Sinigalliano. DETECTION OF HORIZONTAL GENE TRANSFER BY NATURAL TRANSFORMATION IN NATIVE AND INTRODUCED SPECIES OF BACTERIA IN MARINE AND SYNTHETIC SEDIMENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/219 (NTIS PB92195767), 1990.
Description:
Both naturally occurring marine sediments and artificial sediment were used as supports for natural transformation of marine bacteria. hile transformation was not detected in cells of Pseudomonas stutzeri strain ZoBell suspended in artificial seawater, when recipient cells and rifampin resistant DNA were loaded onto sterile sediment columns transformations could be detected at frequencies four to twenty times that for spontaneous resistance. reatment of these columns with Dnase I reduced transformation frequencies to levels comparable to spontaneous resistance frequencies. ransformation was also detected when recipient cells and DNA were loaded on columns prepared from non- sterile sediments, although the frequencies of transformation were lower in these cases than when sterile sediments were employed. Results indicate that marine sediments facilitate the uptake and expression of exogenous DNA by transformable marine bacteria, and that sediments are a more likely niche for natural transformation than the water column in the marine environment.
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DETECTION OF HORIZONTAL GENE TRANSFER BY NATURAL TRANSFORMATION IN NATIVE AND INTRODUCED SPECIES OF BACTERIA IN MARINE AND SYNTHETIC SEDIMENTS