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VERSATILE GAS UPTAKE INHALATION SYSTEM USED IN PHARMACOKINETIC AN METABOLIC STUDIES OF VOLATILE ORGANIC COMPOUNDS
Citation:
McGee, J., M. Ecans, AND W. Crank. VERSATILE GAS UPTAKE INHALATION SYSTEM USED IN PHARMACOKINETIC AN METABOLIC STUDIES OF VOLATILE ORGANIC COMPOUNDS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-96/045, 1995.
Description:
This paper describes an automated gas uptake system for pharmacokinetic research with volatiles, designed to operate within the parameters maintained during conventional whole body inhalation exposures, according to the Good Laboratory Practices (GLP) guidelines. he benefit is to minimize the number of animals required to perform a study, by allowing direct comparison of results from gas uptake studies with results from other inhalation toxicology studies, and by using animals from gas uptake experiments for post-exposure toxicological testing. ther features implemented in the chamber design improve the ease of use and productivity of the system. hese include provisions to adapt the apparatus for use in different pharmacokinetic experimental protocols, automation of data acquisition and oxygen control during exposures, and automated calculation of exposure summary statistics and graphics using a spreadsheet program. he automation system reduces the labor hours required to perform a gas uptake experiment by approximately one-half. ey Words: pharmacokinetics-gas uptake inhalation chambers-gas chromatography-physiological measurements-volatile organic compounds-Good Laboratory Practices (GLP).