Citation:

Miller, S., D. Durall, AND P. Rygiewicz. TEMPORAL ALLOCATION OF 14C TO EXTRAMATRICAL HYPHAE OF ECTOMYCORRHIZAL PONDEROSA PINE SEEDLINGS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/113 (NTIS PB90108424), 1989.

Description:

Ponderosa pine seedlings were inoculated with Hebeloma crustuliniforme either in growth pouches before they were transplanted to root-mycocosms (P seedlings), or at the time of transfer to root-mycocosms (V seedlings). ninoculated seedlings served as controls (U seedlings). he use of root-mycocosms allowed examination of portions of hyphae separate from roots and rooting substrate but still in symbiosis with the host. he results thus provided a quantitative basis for estimating hyphal mass and carbon allocation to extramatrical hyphae. he amount of 14CO2 fixed after a 2-h exposure was greatest for P seedlings and least for inoculated seedlings. our and nine days after exposure, 14C content was greatest in uninoculated seedlings and least in inoculated seedlings. n isotope distribution and dry mass accumulation, V seedlings were more similar to U than to P seedlings. alculated on a dry weight basis, the allocation of isotope to mycelium suggested that extramatrical hyphae of P seedlings were a stronger sink for carbon than extramatrical hyphae of V seedlings. ifferences in inoculation methods resulted in carbon allocation and physiology of extramatrica] hyphae that could affect seedling establishment and survival. eedlings inoculated by one method cannot serve as surrogates for mycorrhizal seedlings produced by other inoculation techniques.

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