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METAL-INDUCED ALTERATION OF THE CELL MEMBRANE/CYTOPLASM COMPLEX STUDIED BY FLOW CYTOMETRY AND DETERGENT LYSIS
Citation:
Zucker, R., K. Elstein, R. Easterling, AND E. Massaro. METAL-INDUCED ALTERATION OF THE CELL MEMBRANE/CYTOPLASM COMPLEX STUDIED BY FLOW CYTOMETRY AND DETERGENT LYSIS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/487 (NTIS PB90185190), 1990.
Description:
Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasm-free ("clean") nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cells membranes/cytoplasm to produce "clean" nuclei. reatment of murine erythroleukemic cells (MELC) with tri-n-butylin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. esistance to NP40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90o scatter and fluorescein isothiocyanate (FITC) fluorescence and was confirmed by light microscropy.
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METAL-INDUCED ALTERATION OF THE CELL MEMBRANE/CYTOPLASM COMPLEX STUDIED BY FLOW CYTOMETRY AND DETERGENT LYSIS