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MICROCOSM AND IN SITU FIELD STUDIES OF ENHANCED BIOTRANSFORMATION OF TRICHLOROETHYLENE BY PHENOL-UTILIZING MICROORGANISMS
Citation:
Hopkins, G., L. Semprini, AND P. McCarty. MICROCOSM AND IN SITU FIELD STUDIES OF ENHANCED BIOTRANSFORMATION OF TRICHLOROETHYLENE BY PHENOL-UTILIZING MICROORGANISMS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/295 (NTIS PN(3222867), 1993.
Description:
The ability of different aerobic groundwater microorganisms to cometabolically degrade trichloroethylene (TCE), 1,2-cis-dichloroethylene (c-DCE), and 1,2-trans-dichloroethylene (t-DCE) was evaluated both in groundwater-fed microcosms and in situ in a shallow aquifer. icrocosms amended with phenol or toluene were equally effective in removing C-DCE (>90%) followed by TCE (60 to 70%), while the microcosm fed methane was most effective in removing T-DCE (>90%). he microcosm fed ammonia was the least effective. one of the microcosms effectively degraded 1,1,1-trichloroethane. t the Moffett Field groundwater test site, in situ removal of C-DCE and TCE coincided with biostimulation through phenol and oxygen injection and utilization, with C-DCE removed more rapidly than TCE. reater TCE and C-DCE removal was observed when the phenol concentration was increased. ver 90% removal of C-DCE and TCE was observed in the 2-m biostimulated zone. his compares with 40 to SO% removal of C-DCE and 15 to 25% removal of TCE achieved by methane-grown microorganisms previously evaluated in an adjacent in situ test zone. he in situ removal with phenol-grown microorganisms agrees qualitatively with the microcosm studies, with the rates and extent of removal ranked as follows: C-DCE > TCE > T-DCE. hese studies demonstrate the potential for in situ TCE bioremediation using microorganisms grown on phenol.