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EFFECT OF AGE ON THE #IN VITRO# PERCUTANEOUS ABSORPTION OF PHENOLS IN MICE
Citation:
Hughes, M., H. Fisher, L. Birnbaum, AND L. Hall. EFFECT OF AGE ON THE #IN VITRO# PERCUTANEOUS ABSORPTION OF PHENOLS IN MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/528 (NTIS PB95148904), 1994.
Description:
The effect of age on the in vitro dermal absorption of phenol, cyanophenol, acetamidophenol and heptyloxyphenol was examined. kin from pre-clipped male C57BL/6N mice of ages 3. 15 and 27 months was mounted in flow-through diffusion cells. [14C]-phenol and analogs (4 ug/cm2) were applied to the skin (0.32 cm2) in ethanol (5 ul) and absorption was measured under occluded conditions for 72 hr. ignificant age effects on cumulative absorption were found with phenol and heptyloxyphenol. bsorption of these two compounds into the receptor fluid was significantly greater in 27 month old mice compared to the younger animals. n addition, phenol absorption in 15 month old mice was significantly greater than 3 month old mice. owever, the differences in phenol absorption were small (<5%) and may not be biologically significant. he maximal rates of absorption of cyanophenol and phenol were affected by age. he maximal rate for cyanophenol absorption was significantly lower in 15 month old mice than the rate obtained for 3 and 27 month old animals. he maximal rate for phenol absorption was significantly greater in 27 month old mice compared to the younger animals. he overall effect of age on the in vitro dermal absorption of phenols using skin from C57BL/6N mice appears to be compound dependent.
