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IN VITRO LYMPHOCYTE PROLIFERATION ASSAYS: THE MITOGEN-STIMULATED RESPONSE AND THE MIXED LYMPHOCYTE REACTION IN IMMUNOTOXICITY TESTING
Citation:
Smialowicz, R. IN VITRO LYMPHOCYTE PROLIFERATION ASSAYS: THE MITOGEN-STIMULATED RESPONSE AND THE MIXED LYMPHOCYTE REACTION IN IMMUNOTOXICITY TESTING. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-91/284 (NTIS PB92126911), 1991.
Description:
This report described detailed methodologies for the determination of rho in vitro mitogen-stimulated response and one-way mixed lymphocyte reaction of mouse and rat lymphocytes. ist of reagents, supplies and equipment necessary for the successful completion of these assays is provided. lso, a step-by-step description of each of these assays is presented, so that laboratories unfamiliar with these procedures should be able to perform them. hese assay's are useful for the identification and characterization of agents capable of altering the immune system and as such are useful as screening tests for potential immunotoxicants.