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INFLUENCE OF ROOT COLONIZING BACTERIA ON THE DEFENSE RESPONSES OF BEAN
Citation:
Zdor, R. AND A. Anderson. INFLUENCE OF ROOT COLONIZING BACTERIA ON THE DEFENSE RESPONSES OF BEAN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/221 (NTIS PB93205045), 1992.
Description:
Colonization of plant roots by fluorescent pseudomonads has been correlated with disease suppression. ne mechanism may involve altered defense responses in the plant upon colonization. ltered defense responses were observed in bean (Phaseolus vulgaris) inoculated with fluorescent pseudomonads. ystemic effects of root inoculation by Pseudomonas putida isolate Corvallis. SDS-polyacrylamide gel electrophoresis demonstrated that levels of certain acid-soluble proteins increased in the leaf extracts of inoculated plants. lants inoculated with REW1-1-1 produced more of a 57 M. protein, and plants inoculated with isolates P9A and REW11-1-1 produced more of a 38 M. protein. orthern hybridization revealed enhanced accumulation of mRNAs, that encode the pathogenesis-related protein. R1a in leaves of plants inoculated with P. putida and REW1-1-1-1. henolics and photoazlexins accumulated in bean cotyledons exposed to REW1-1-1 for 24 h but little change in levels of these compounds occurred in cotyledons inoculated with P9A and P. putida. oth suspension culture cells and roots treated with REW1-1-1 rapidly evolved more hydrogen peroxide than those exposed to P9A and P. putida. owever, roots from 14-day-old-plants colonized by P9A, P. putida or REW1-1-1-1 did not have higher levels of phenolics, phytolexins or mRNAs for two enzymes involved in phenolic biosynthesis, phenylalanine-ammonia lyase and chalcone synthase. elective induction of plant defense strategies upon root colonization by certain pseudomonads is present.