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CLONING AND CHARACTERIZATION OF TFDS, THE REPRESSOR-ACTIVATOR GENE OF TFDB FROM THE 2,4-DICHLOROPHENOXYACETIC ACID CATABOLIC PLASMID PJP4
Citation:
Kaphammer, B. AND R. Olsen. CLONING AND CHARACTERIZATION OF TFDS, THE REPRESSOR-ACTIVATOR GENE OF TFDB FROM THE 2,4-DICHLOROPHENOXYACETIC ACID CATABOLIC PLASMID PJP4. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/552 (NTIS PB92129634), 1990.
Description:
Plasmid pR101 inducible for 2,4-dichlorophenol hydroxylase (DCPH) encoded by tfdB. lasmid pRO103 has elevated basal levels of DCPH but is uninducible. he regulatory gene for tfdB, designated tfdS, was cloned as an 8.3 kbp EcorRI-E fragment. hen the cloned tfdS gene was in trans with plasmid pRO103, baseline DCPH levels were repressed to normal uninduced levels and fully induced when grown in presence of 2,4-dichlorophenoxyacetic acid, 2,4-dichlorophenol, or 4-chloro-catechol (4CC). owever, when tfdS was in trans with tfdB, in absence of tfdCDEF, tfdB was repressed but could not be induced. hen tfdS and tfdCl are in trans with tfdB, tfdB remained uninduced, indicating that a downstream metabolite of chloro-cis, cis-muconate, either 2-cis-chlorodiene lactone or chloromaleyacetic acid, is the effector. ata demonstrate that the gene product of tfdS acts as a repressor of tfdB in the absence of an effector and as an activator of tfdB when an effector is present.
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CLONING AND CHARACTERIZATION OF TFDS, THE REPRESSOR-ACTIVATOR GENE OF TFDB FROM THE 2,4-DICHLOROPHENOXYACETIC ACID CATABOLIC PLASMID PJP4