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ANALYTICAL VARIATION IN THE DETERMINATION OF THE FATTY ACID COMPOSITION OF STANDARD PREPARATIONS OF BRINE SHRIMP ARTEMIA: AN INTERLABORATORY EXERCISE
Citation:
Leger, P., D. Bengtson, AND P. Sorgeloos. ANALYTICAL VARIATION IN THE DETERMINATION OF THE FATTY ACID COMPOSITION OF STANDARD PREPARATIONS OF BRINE SHRIMP ARTEMIA: AN INTERLABORATORY EXERCISE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/148 (NTIS PB90125766), 1989.
Description:
An international interlaboratory exercise was conducted to investigate the variability associated with the preparation and analysis of samples and the reporting of fatty acid composition data for tvo samples of Artemia supplied to the laboratories by the Artemia Reference Center (ARC). Results reported to the ARC indicated that intralaboratory variability in determinations of total lipid content and fatty acid composition by the area-percent method vas generally low, with a coefficient of variation (CV) around 5 to 7% on average. lntralaboratory variability in determination of fatty acid composition by the mg/g method was higher, with CVs greater than 10% in several cases. lntralaboratory variability was generally much greater, with CVs of greater than 2O% for total lipid content, 8 to 27% for composition of individual fatty acids determined by the area percent method, and 12 to 49% for composition of individual fatty acids determined by the mg/g method, Thus, the precision of the data from any given laboratory is generally adequate, but the accuracy may not be. Nevertheless, in this case, data from each of the laboratories would support the decision to use these particular Artemia only for freshwater organisms.