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EFFECTS OF 2,4-DICHLOROPHENOL, A METABOLITE OF A GENETICALLY ENGINEERED BACTERIUM, AND 2,4-DICHLOROPHENOXYACETATE ON SOME MICROORGANISM-MEDIATED ECOLOGICAL PROCESSES IN SOIL
Citation:
Short, K., J. Doyle, R. King, R. Seidler, G. Stotzky, AND R. Olsen. EFFECTS OF 2,4-DICHLOROPHENOL, A METABOLITE OF A GENETICALLY ENGINEERED BACTERIUM, AND 2,4-DICHLOROPHENOXYACETATE ON SOME MICROORGANISM-MEDIATED ECOLOGICAL PROCESSES IN SOIL. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/144 (NTIS PB91219022), 1991.
Description:
A genetically engineered microorganism, Pseudomonas putida PPO301 (pRO103), and the plasmidless parent strain, PPO301, were added at approximately 10 7 CFU/g of soil amended with 500 ppm of 2,4-dichlorophenoxyacete (2,4-D)(500 ug/g). he degradation of 2,4-D and the accumulation of a single metabolite, identified by gas chromatography-mass spectrophotometry as 2, 4-dichlorophenol (2,4-DCP), occurred only in soil inoculated with PPO301 (pRO103), wherein 2,4-DCP accumulated to >70 ppm for 5 weeks and the concentration of 2,4-D was reduced to <100 ppm. oincident with the accumulation of 2,4-DCP was a >400-fold decline in the numbers of fungal propagules and a marked reduction in the rate of CO2 evolution, whereas 2,4-D did not depress either fungal propagules or respiration of the soil microbiota. ,4-DCP did not appear to depress the numbers of total heterotrophic, sporeforming, or chitin-utilizing bacteria. n vitro and in situ assays conducted with 2,4-DCP and fungal isolates from the soil demonstrated that 2,4-DCP was toxic to fungal propagules at concentrations below those detected in the soil.
