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KERATINOCYTE CELL-MEDIATED MUTAGENESIS ASSAY: CORRELATION WITH IN VIVO TUMOR STUDIES
Citation:
Reiners, Jr., J., L. Yotti, C. McKeown, S. Nesnow, AND T. Slaga. KERATINOCYTE CELL-MEDIATED MUTAGENESIS ASSAY: CORRELATION WITH IN VIVO TUMOR STUDIES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-83/228 (NTIS PB84232719), 1983.
Description:
A murine keratinocyte cell-mediated mutagenesis assay was characterized and examined as an in vitro model system for studying the biotransformation of promutagens/procarcinogens by mouse skin. The assay used living cultured newborn SENCAR keratinocytes for the metabolic activation of promutagens and Chinese hamster lung V-79 fibroblasts for detection of resulting mutagens. Mutations at, or affecting, the hypoxanthineguanine phosphoribosyltransferease locus were scored by resistance to 6-thioguanine. The relative mutagenicities of several polycyclic aromatic hydrocarbons (PAHs) in the cell-mediated assay correlated with the in vivo skin tumorigenicity of the PAHs determined in a two-stage carcinogenesis protocol.