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CHARACTERISTICS OF IS401, A NEW MEMBER OF THE IS3 FAMILY IMPLICATED IN PLASMID REARRANGEMENTS IN PSEUDOMONAS CEPACIA
Citation:
Byrne, A. AND T. Lessie. CHARACTERISTICS OF IS401, A NEW MEMBER OF THE IS3 FAMILY IMPLICATED IN PLASMID REARRANGEMENTS IN PSEUDOMONAS CEPACIA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/447 (NTIS PB95112124), 1994.
Description:
We have determined the nucleotide sequence of IS401, an insertion sequence implicated in rearrangements of a 170-kb cryptic plasmid from Pseudomonas cepacia. ur analysis focused on a 4066-bp plasmid fragment containing adjacent copies of IS401 and of IS408, an element reported previously to activate gene expression in P. cepacia. ne objective was to determine if an apparent increase in the copy number of IS401 in strains carrying adjacent plasmid copies of these two elements might be due to readthrough transcription of an IS401 transposase gene from an outwardly directed promoter within IS408. his possibility was ruled out by nucleotide sequence analysis of the 4066-bp plasmid fragment, which indicated that the major open reading frames of IS401 were oriented in the direction of IS408. S401 was 1316 bp in length and had 26-bp terminal inverted repeats flanked b 3-bp direct duplications of adjacent DNA. t was closely related to the 133 family elements S51 from P. savastanoi and IS3411 from Escherichia coli. ertinent features of IS408 are also discussed.
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CHARACTERISTICS OF IS401, A NEW MEMBER OF THE IS3 FAMILY IMPLICATED IN PLASMID REARRANGEMENTS IN PSEUDOMONAS CEPACIA