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MEASURING THE DISPERSAL AND REENTRAINMENT OF RECOMBINANT PSEUDOMONAS SYRINGAE AT CALIFORNIA TEST SITES
Citation:
Seidler, R., M. Walters, S. Hern, V. Fieland, D. Schmedding, AND S. Lindow. MEASURING THE DISPERSAL AND REENTRAINMENT OF RECOMBINANT PSEUDOMONAS SYRINGAE AT CALIFORNIA TEST SITES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/395, 1994.
Description:
The dispersal of genetically engineered Pseudomonas syringae and Pseudomonas fluorescens was investigated during and after spray applications onto plants at Brentwood and Tulelake, California. ive different sampling devices were used to evaluate the dispersal within and around test plots. hese included six-stage Andersen, Reynier slit, and all glass impinger samplers, gravity settling plates, and sentinel plants. esults indicated that over 99.99 percent of the 10 12 sprayed bacteria settled directly onto the soil and plants in the test plot. evertheless, during and immediately after the spray, 10 6 viable cells were detected within 15m to 30m from the site of the spray. he spread of small numbers of microbes away from test plot during aerosol applications or by entrainment must be expected even under calm wind conditions.