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INSECT VIRUS: ASSAYS FOR VIRAL REPLICATION AND PERSISTENCE IN MAMMALIAN CELLS
Citation:
Hartig, P., M. Cardon, AND C. Kawanishi. INSECT VIRUS: ASSAYS FOR VIRAL REPLICATION AND PERSISTENCE IN MAMMALIAN CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/126 (NTIS PB91211490), 1991.
Description:
Viral pesticidal agents must be evaluated for potential health hazards prior to utilization. ssessment of the likelihood of replication in humans has included in vitro exposure of human cells to the potential pesticidal agent. revious in vitro evaluation strategies have lacked positive controls. hus, negative results, interpreted as no effect of the virus on human cells, could reflect basic deficiencies in the testing protocols. e have designed a testing scheme for viral pesticides and utilized it to test the nuclear polyhedrosis virus of Autographa californica. ests were aimed at evaluating potential replication or gene expression in primate cells. arallel tests were run utilizing identical protocols with primate viruses known to produce the biological effect being evaluated. hus, protocols described herein have been tested with positive viral controls.