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RAPID EFFICIENT PRODUCTION OF BACULOVIRUS EXPRESSION VECTORS
Citation:
Hartig, P. AND M. Cardon. RAPID EFFICIENT PRODUCTION OF BACULOVIRUS EXPRESSION VECTORS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/363 (NTIS PB93228898), 1992.
Description:
Recombinant baculoviruses have been utilized to produce foreign proteins and have the potential to be safe, efficacious insecticides. solation of recombinant virus is usually by plaque phenotype. ypical recombination rates are less than 1%, thus requiring time consuming inspection of hundreds of individual plaques. ere we describe a method of generating recombinants which requires less time than current protocols and frequently produces recombinants at rates exceeding 30%. his protocol employs liposome mediated transfection, reduced post-transfection incubation times, linearized parental virus which produces occlusion positive plaques in clones of the parental genotype, and colorimetric detection of recombinants. his protocol allows the initial, and frequently the final, isolation of recombinants in 7 calendar days.
