You are here:
BIASED DNA INTEGRATION IN COLLETOTRICHUM GLOEOSPORIOIDES F. SP. AESCHYNOMENE TRANFORMANTS WITH BENOMYL RESISTANCE
Citation:
Armstrong, J. AND D. Harris. BIASED DNA INTEGRATION IN COLLETOTRICHUM GLOEOSPORIOIDES F. SP. AESCHYNOMENE TRANFORMANTS WITH BENOMYL RESISTANCE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/256 (NTIS PB93212629), 1993.
Description:
A procedure is presented for transforming Colletotrichum gloeosporioides f. sp. aeschynomene (Cga) to benomyl by using a mutant B-tubulin gene from Neurospora crassa. ybridization between the N. crassa B-tubulin gene and transformant DNAs digested with Sty I indicated that the integration site in all transformants was in a specific region of the genome. ransformants tolerated up to 300 ug penomyl/ml, but differed in pigmentation, growth rate, and hytopathology. ne of the transformants and the parent strain exhibited similar survival in microcosms containing crimson clover plants and soil. NA of benomyl isolates recovered from the microcosms after 15 d contained the benomyl gene on the same location as the train that was sprayed on the plants and soil. e speculate that the bias in the site of integration was due to selection against transformants with other configurations between the N. crassa 13-tubulin gene and Cga genome, which were unstable, lethal, or unsuitable for expression of the ben phenotype.