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SURVIVAL DIFFERENCES AMONG FREEZE-DRIED GENETICALLY ENGINEERED AND WILD-TYPE BACTERIA
Citation:
Israeli, E., B. Shaffer, J. Hoyt, B. Lighthart, AND L. Ganio. SURVIVAL DIFFERENCES AMONG FREEZE-DRIED GENETICALLY ENGINEERED AND WILD-TYPE BACTERIA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/255 (NTIS PB93212611), 1993.
Description:
Spray application is often used to introduce genetically engineered microorganisms into the environment. he risk associated with the downwind transport and survival necessitates development of tools to assess the risk associated with their airborne transport. ecause the death mechanism of bacteria that occurs during freeze-drying and spray aerosolization is thought to be similar, establishing death rates of freeze-dried bacteria might serve as a tool to assess the risk associated with their airborne survival and transport. o this end, engineered strains of Escherichia coli and Pseudomonas syringas were freeze-dried and exposed to air, visible light, or both. ll engineered strains were found to have significantly higher rates than their parental strains. ight and air exposure were found to have an enhanced effect on the death rates of all strains. pplication of freeze-dried engineered bacteria death rate models for release into the environment is discussed.