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IMPROVED SEA URCHIN DNA-BASED EMBRYO GROWTH TOXICITY TEST
Citation:
Jackim, E. AND D. Nacci. IMPROVED SEA URCHIN DNA-BASED EMBRYO GROWTH TOXICITY TEST. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/266 (NTIS PB87167144), 1986.
Description:
The article presents an improved DNA-based embryo toxicity test in which growth is measured as the increase in DNA during a 5-h postfertilization period. The test, using direct fluorometric determination of DNA rather than thymidine incorporation, is fast, sensitive, easy to conduct, and correlates well with other 48- and 96-h acute aquatic tests. The need for radioisotopes has been eliminated and data variability is substantially reduced. It is recommended that this test be incorporated into a battery of rapid tests used to estimate aquatic toxicity.
Record Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:12/31/1986
Record Last Revised:12/22/2005
Record ID:
46086