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CONTINUOUS, ON-LINE INTERFACE FOR REVERSE-PHASE MICROBORE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/DIFFUSE REFLECTANCE INFRARED FOURIER TRANSFORM ANALYSIS
Citation:
Castles, M., L. Azarraga, AND L. Carreira. CONTINUOUS, ON-LINE INTERFACE FOR REVERSE-PHASE MICROBORE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/DIFFUSE REFLECTANCE INFRARED FOURIER TRANSFORM ANALYSIS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/183 (NTIS PB87116463), 1986.
Description:
An ultrasonic nebulizer with a piezo-electric transducer and a newly designed and constructed continuous on-line collection station have been adapted as an interface between a reverse-phase microbore high-performance liquid chromatograph (RP (mu)HPLC) and a diffuse reflectance infrared Fourier transform spectrometer. The collection station incorporated a directional vacuum port for confinement of the nebulized material onto a small area (3 to 5 mm i.d.) of the diamond powder. The inherent desolvation that occurs upon nebulization of the reverse-phase eluant, along with the vacuum collection system and the diamond powder diffuse reflectance substrate, makes possible the continuous on-line collection and FT-IR analysis of the compounds separated by a RP-(mu)HPLC without any type of initial solute extraction. (Copyright (c) 1986 Society for Applied Spectroscopy.)