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CHARACTERIZATION OF SULFHYDRYL PROTEINS INVOLVED IN THE MAINTENANCE OF FLAGELLAR STRAIGHTNESS IN HAMSTER SPERMATOZOA
Citation:
Cornwall, G. AND T. Chang. CHARACTERIZATION OF SULFHYDRYL PROTEINS INVOLVED IN THE MAINTENANCE OF FLAGELLAR STRAIGHTNESS IN HAMSTER SPERMATOZOA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/239 (NTIS PB91116996), 1990.
Description:
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). lagellar bending is prevented by oxidizing sperm sulfhydryl (SH) groups with diamide. n the present study, the authors examined the SH proteins involved in a sperm flagellar bending using two-dimensional polyacrylamide gel electrophoresis and monobromobimane, and SH-specific fluorescent dye. roteins extracted from samples containing bent caput spermatozoa contained more reduced SH than the same proteins extracted from straight caput spermatozoa. o further characterize these sperm SH proteins, caudal epididymal spermatozoa that exhibited straight flagella were induced to undergo flagellar bending by treatment with the SH reductant dithiothreitol. pecific proteins of 85-94 kDa and 35-40 kDa, which were rich in SH groups when extracted from samples containing bent caput spermatozoa, were also SH-rich.
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CHARACTERIZATION OF SULFHYDRYL PROTEINS INVOLVED IN THE MAINTENANCE OF FLAGELLAR STRAIGHTNESS IN HAMSTER SPERMATOZOA