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EVALUATION OF THE IMMUNOTOXICITY OF ORALLY ADMINISTERED 2-METHOXYACETIC ACID IN FISCHER 344 RATS
Citation:
Smialowicz, R., M. Riddle, R.R. Rogers, C. Copeland, R. Luebke, AND D. Andrews. EVALUATION OF THE IMMUNOTOXICITY OF ORALLY ADMINISTERED 2-METHOXYACETIC ACID IN FISCHER 344 RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/304 (NTIS PB92124601), 1991.
Description:
We previously demonstrated that the glycol ether 2-methoxyethanol (ME) immunotoxic in the rat. n this study, the immunotoxicity of 2-methoxyacetic acid (MAA), the principal metabolite of ME, was evaluated in adult male Fischer 3 rats. ats were dosed by gavage with MAA on ten consecutive days at dosages ranging from 50 to 200 mg/kg/d MAA. hymic involution, in the absence of body weight loss, was observed at 100 and 200 mg/kg/d MAA. ymphoproliferative responses to the mitogens concanavalin A (Con A), phytohemag-glutinin (PHA) and pokeweed mitogen (PWM) were also reduced at these dosages. he in vitro generated cytotoxic T lymphocyte response was reduced at 200 g/kg/d MAA. he mixed lymphocyte reaction (MLR) and natural killer (NK) cell activity were unaffected by exposure to MAA. numeration of splenic lymphocyte populations revealed a reduction in the percentage of W3/25-positive cells at 100 and 150 mg/kg/d and an increase in the percentage of OX39 positive cells at 200 mg/kg/d; however, no changes in the absolute number of either of these subsets were observed. he plaque forming cell (PFC) response to trinenyl-lipopolysaccharide (TNP-LPS) was suppressed at 50-200 mg/kg/d hile the PFC response to sheep red blood cells (SRBC) was elevated at 50 mg/kg/d.