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IN VITRO MAMMALIAN MUTAGENESIS AS A MODEL FOR GENETIC LESIONS IN HUMAN CANCER
Citation:
Hozier, J., M. Applegate, AND M. Moore. IN VITRO MAMMALIAN MUTAGENESIS AS A MODEL FOR GENETIC LESIONS IN HUMAN CANCER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/448 (NTIS PB93141471), 1992.
Description:
Recently, in vitro mammalian cell assays of mutagenesis have been criticized as being poorly predictive of long-term in vivo rodent assays of carcinogenicity. Yet in vitro as says using mammalian cells might be expected to register types of genetic lesions thought to be important to human malignant disease. olecular and cytogenetic analysis of mutations induced by a variety of genotoxic compounds at the heterozygous thymidine kinase locus ip mouse lymphoma cells indicates that this in vitro assay does indeed register the range of genetic lesions recently found `in a wide variety of human tumors. he types and complexity of the induced lesions are reflected in mutant cell colony phenotype in a compound-specific fashion. hese studies point to the use of appropriate in vitro mammalian mutagenesis assays as new model systems for dissecting the genetic lesions important to human carcinogenesis and as a means of determining the potential for compounds to induce such lesions.