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CHARACTERIZATION OF THE ALKANE-INDUCIBLE CYTOCHROME P450 (P450ALK) GENE FROM THE YEAST CANDIDA TROPICALIS: IDENTIFICATION OF A NEW P450 GENE FAMILY
Citation:
Sanglard, D. AND J. Loper. CHARACTERIZATION OF THE ALKANE-INDUCIBLE CYTOCHROME P450 (P450ALK) GENE FROM THE YEAST CANDIDA TROPICALIS: IDENTIFICATION OF A NEW P450 GENE FAMILY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/304 (NTIS PB93229268), 1989.
Description:
The P450ALK gene, which is inducible by the assimilation of alkane in Candida tropicalis, was sequenced and characterized. tructural features described in promoter and terminator regions of Saccharomyces yeast genes are present in the P450alk gene and some particular structures are discussed for their possible role in the inducibility of this gene. xpression of the P450alk gene was achieved in Saccharomyces cerevisiae using the yeast alcohol dehydrogenase expression system after removal of the P450alk gene flanking regions. he resultant expressed protein had a molecular mass slightly greater than that of P450alk from C. tropicalis . This alteration did not prevent the function and the localization of P450alk expressed in S. cerevisiae, as this organism showed an acquired microsome-bound activity for the terminal hydroxylation of lauric acid. he deduced P450alk amino acid sequence was compared with members of the nine known P450 gene families. hese comparisons indicated that P450alk had a low relationship with these members and was therefore the first member (Al) of a new P450 gene family (LII).
