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DETERMINATION OF GIARDIA LAMBLIA CYST INFECTIVE DOSE FOR THE MONGOLIAN GERBIL (MERIONES UNQUICULATUS)
Citation:
Schaefer, F., C. Johnson, C. Hsu, AND E.W. Rice. DETERMINATION OF GIARDIA LAMBLIA CYST INFECTIVE DOSE FOR THE MONGOLIAN GERBIL (MERIONES UNQUICULATUS). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/171 (NTIS PB91231399), 1991.
Description:
The purpose of this study was to determine the I.D.50 for Giardia lamblia (CDC:0284:1) cysts in Mongolian gerbils (Meriones unquiculatus) and compare it to human infectivity data. ysts were purified from Mongolian gerbil feces and diluted to produce inocula for each dosage group. fter observation of the gerbils for production of either cysts in the feces or trophozoites in the small intestine over a two week period, the I.D.50 results calculated by Probit analysis, Spearman-Karber, and Reed-Muench were 282, 288, and 219 cysts, respectively. east squares regression analysis, relating the number of cysts inoculated and the percent of infectivity, estimated the log 10 I.D.50 to be 2.50,with a coefficient of correlation (r) of 0.9689. he log 10 I.D.50 derived by this method is similar to those calculated by the previous methods. ince human feeding studies indicate the I.D.50 for humans is < 10 and our results indicate the I.D.50 for gerbils to be around 285 cysts, the gerbil model can not be trusted to reflect what might happen in human exposures. EPA/600/R-93/014
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DETERMINATION OF GIARDIA LAMBLIA CYST INFECTIVE DOSE FOR THE MONGOLIAN GERBIL (MERIONES UNQUICULATUS)