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EFFECTS OF GLYPHOSATE AND NITRAPYRIN ON SELECTED BACTERIAL POPULATIONS IN CONTINUOUS-FLOW CULTURE
Citation:
Hendricks, C. AND A. Rhodes. EFFECTS OF GLYPHOSATE AND NITRAPYRIN ON SELECTED BACTERIAL POPULATIONS IN CONTINUOUS-FLOW CULTURE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/401 (NTIS PB93121309), 1992.
Description:
This study examines the effects of Roundup [N-(phosphonomethyl)glycine] and N-Serve [2-chloro-6-(trichloromethyl)pyridine] on the culture of soil organisms in a continuous-flow column system. n this study, nitrifying and various heterotrophic bacterial populations were enumerated over a 16-day period. he chemicals Roundup and N-Serve were added at day 8. eterotrophic bacterial populations increased significantly in columns with 4.2 mg nitrapyrin and 68 mg glyphosate g-1 dry soil. umbers of heterotrophs were not significantly different from controls in columns at lower chemical concentrations. umbers of nitrifying bacteria did not appear to change following treatment, although nitrification was inhibited. luorescent antibody analysis of the nitrifying bacteria revealed that Nitrosolobus was more numerous than Nitrosospira and Nitrosomonas. itrosolobus increased in number, whereas the other two genera remained unchanged. n this study, the continuous-flow system proved to be both reliable and useful in the culture of nitrifying bacteria. his method is an alternative to traditional techniques for evaluation of the effects of chemicals on microbial biogeochemical cycles.