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IMPROVED FLOW CYTOMETRIC ASSAY FOR SOMATIC MUTATIONS AT THE GLYCOPHORIN A LOCUS IN HUMANS
Citation:
Langlois, R., B. Nisbet, W. Bigbee, D. Ridinger, AND R. Jensen. IMPROVED FLOW CYTOMETRIC ASSAY FOR SOMATIC MUTATIONS AT THE GLYCOPHORIN A LOCUS IN HUMANS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/229 (NTIS PB91117093), 1990.
Description:
An improved method has been developed for the glycophorin A assay for somatic cell mutations in humans. he new assay, named the "BR6" assay, can be performed on a commercially available, single-beam flow cytometer, in contrast to the previously described 1W1 assay that required a dual-beam flow sorter. odified cell labeling method developed for the BR6 assay results in improved separation of normal and mutant phenotype cells, as well as eliminating some cellular artifacts that affected the 1W1 assay. arallel measurements on samples from 17 normal donors showed that the BR6 assay yields comparative variant cell frequencies and improved measurement precision compared with 1W1 assay. etailed analysis of three individuals who showed large differences in background variant frequency with the 1W1 assay confirmed that these differences could also be detected with the BR6 assay for an individual exposed to a high level of ionizing radiation in an accident at Goiania, Brazil.
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IMPROVED FLOW CYTOMETRIC ASSAY FOR SOMATIC MUTATIONS AT THE GLYCOPHORIN A LOCUS IN HUMANS