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INDUCTION OF 6-THIOGUANINE RESISTANCE IN SYNTHRONIZED HUMAN FIBROBLAST CELLS TREATED WITH METHYL METHANESULFONATE, N-ACETOXY-2-ACETHYLAMINOFLUORENE AND N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
Citation:
Huang, S., S. Huang, C. Casperson, AND M. Waters. INDUCTION OF 6-THIOGUANINE RESISTANCE IN SYNTHRONIZED HUMAN FIBROBLAST CELLS TREATED WITH METHYL METHANESULFONATE, N-ACETOXY-2-ACETHYLAMINOFLUORENE AND N-METHYL-N'-NITRO-N-NITROSOGUANIDINE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-81/181 (NTIS PB83209726), 1981.
Description:
Chemical induction of 6-thioguanine resistance was studied in synchronized human fibroblast cells. Cells initially grown in a medium lacking arginine and glutamine for 24 h ceased DNA synthesis and failed to enter the S phase. After introduction of complete medium, the cells progressed to the S phase after 16 h. DNA synthesis peaked 20 h after removal of nutrient stress and declined. Mutations were induced in S-phase cells by methyl methanesulfonate (MMS), N-acetoxy-2-acetylaminofluorene (NA-AAF) and N-methyl-N nitro-N-nitrosoguanidine (MNNG). Chemical treatments resulted in an increase in the absolute number of mutant colonies and in a dose-dependent mutation frequency.