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MUTANTS OF PSEUDOMONAS CEPACIA G4 DEFECTIVE IN CATABOLISM OF AROMATIC COMPOUNDS AND TRICHLOROETHYLENE
Citation:
Shields, M., S. Montgomery, S. Cuskey, P. Chapman, AND P. Pritchard. MUTANTS OF PSEUDOMONAS CEPACIA G4 DEFECTIVE IN CATABOLISM OF AROMATIC COMPOUNDS AND TRICHLOROETHYLENE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/337 (NTIS PB92129741), 1991.
Description:
Pseudomonas cepacia strain G4 possesses a novel pathway of toluene catabolism that is shown to be responsible for the degradation of trichloroethylene (TCE). his pathway involves conversion of toluene via o-cresol to 3-methylcatechol. o determine the enzyme f toluene degradation that is responsible for TCE degradation, chemically induced mutants, blocked in toluene utilization, were examined. utants of a class designated tomA were all defective in their ability to oxidize toluene o-cresol, m-cresol and phenol, suggesting that a single enzyme is responsible for conversion of these compounds to their hydroxylated products (3-methylcatechol from toluene, o-cresol, and m-cresol, and catechol from phenol) in the wild type. utants of this class did not degrade TCE. wo other mutant classes blocked in toluene catabolism, tomC, lacking catechol-2,3-dioxygenase or tomC, lacking 2-hydroxy-6-oxoheptadienoic acid hydrolase activity, were fully capable of TCE degradation. herefore, TCE degradation is directly associated with the monooxygenation capability responsible for toluene, cresol and phenol hydroxylation.
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MUTANTS OF PSEUDOMONAS CEPACIA G4 DEFECTIVE IN CATABOLISM OF AROMATIC COMPOUNDS AND TRICHLOROETHYLENE