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SEPARATION OF 32P-LABELED 3',5'-BISPHOSPHATE NUCLEOTIDES OF POLYCYCLIC AROMATIC HYDROCARBON ANTI-DIOL-EPOXIDES AND DERIVATIVES
Citation:
Zhan, D., D. Herreno-Saenz, L. Chiu, L. Tungeln, Y. Wu, J. Lewtas, AND P. Fu. SEPARATION OF 32P-LABELED 3',5'-BISPHOSPHATE NUCLEOTIDES OF POLYCYCLIC AROMATIC HYDROCARBON ANTI-DIOL-EPOXIDES AND DERIVATIVES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-96/041, 1995.
Description:
23P-Postlabeling/HPLC is a highly sensitive analytical method for identification of chemical-modified DNA adducts isolated from experimental animals and human samples. o determine the optimal 32P-postlabeling/HPLC conditions for efficient separation, we employed ten diol-epoxide-modified 3,5'-bisphospate deoxynucleotides derived from benzo[a]pyrene (BaP),nitrated BaP, and related compounds. fter evaluating ODS-modified, C4-modified, phenyl-modified, diphenyl-modified, and cyclobonded reversed-phase HPLC columns, we found that the Vydac diphenyl-modified column can efficiently separate these 31, 51-bisphosphate deoxynucleotides. he results suggest that 32P-postlabeling/HPLC is a useful methodology for detecting environmental carcinogens that can be metabolized to diol-epoxides. he relationships between the structures of anti-diol-epoxides and HPLC retention order are also discussed.