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IN VITRO PERCUTANEOUS APPROACH OF SODIUM ARSENATE IN B6C3F1 MICE
Citation:
Rahman, M., L. Hall, AND M. Hughes. IN VITRO PERCUTANEOUS APPROACH OF SODIUM ARSENATE IN B6C3F1 MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/120, 1994.
Description:
Percutaneous absorption of sodium [73As] arsenate in female B6C3F1 mice was investigated in this study from various exposure conditions, including solid compound, aqueous solution (100 and 250 ul) and soil (= 23 mg/cm2). In vitro diffusion experiments were conducted for 24 hr using previously clipped full-thickness dorsal skin in a flow-through system with Hepes-buffered Hanks; balanced salt solution as the receptor fluid. Doses of 5, 50, 500 and 5000 ng were applied to the skin surface (area = 0.64 cm2) in the aforementioned vehicles and dermal absorption was quantitated by summing the amounts of arsenate-derived radioactivity in the receptor fluid and skin following washing of the skin surface to remove unpenetrated compound. Absorption of sodium arsenate increased linearly with the applied dose from all exposure vehicles, with a constant fraction of the dose being absorbed. Maximum absorption (62% of the applied dose) was obtained from the 100 ul aqueous vehicle and the skin had a higher content of the compound than the receptor fluid. Soil provided the least (<0.3% of the applied dose) absorption of the chemical, with almost all of the absorbed dose residing within the skin. Even short-term (1 hr) dermal exposure of arsenate in water resulted in the passage of the chemical into the skin, which upon further perfusion (23 hr), passed into the receptor fluid. Thus, exposure vehicle plays an important role on the in vitro dermal absorption of sodium arsenate in B6C3F1 mice, with the aqueous vehicle providing greater absorption than the soil.