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PHYLOGENETIC TREE OF 16S RIBOSOMAL RNA SEQUENCES FROM SULFATE-REDUCING BACTERIA IN A SANDY MARINE ENVIRONMENT
Citation:
Devereaux, R. AND G. Mundfrom. PHYLOGENETIC TREE OF 16S RIBOSOMAL RNA SEQUENCES FROM SULFATE-REDUCING BACTERIA IN A SANDY MARINE ENVIRONMENT. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/436 (NTIS PB95111829), 1994.
Description:
Phylogenetic divergence among sulfate-reducing bacteria in an estuarine sediment sample was investigated by PCR amplification and comparison of partial 16S rDNA sequences. wenty unique 16S RDNA sequences were found, 12 from delta subclass bacteria based on overall sequence similarity (82-91%). wo successive PCR amplifications were used to obtain and clone the 16S rDNA. he first reaction used templates derived from phosphate-buffered saline washed sediments with primers designed to amplify nearly full-length bacterial domain 16S rDNA. roduce from a first reaction was buffered saline washed sediment used as template in a second reaction with primers designed to selectively amplify a region of 16S rRNA genes of sulfate-reducing bacteria. hylogenetic tree incorporating the cloned sequences suggests the presence of yet to be cultivated lines of sulfate-reducing bacteria within the sediment sample.
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PHYLOGENETIC TREE OF 16S RIBOSOMAL RNA SEQUENCES FROM SULFATE-REDUCING BACTERIA IN A SANDY MARINE ENVIRONMENT