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INTESTINAL SURVIVAL, COMPETITION AND TRANSLOCATION OF BIOTECHNOLOGY AGENTS UPON INTRANASAL EXPOSURE OF C3H/HEJ MICE
Citation:
George, S., M. Kohan, M. Taylor, H. Brooks, J. Creason, AND L. Claxton. INTESTINAL SURVIVAL, COMPETITION AND TRANSLOCATION OF BIOTECHNOLOGY AGENTS UPON INTRANASAL EXPOSURE OF C3H/HEJ MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/401 (NTIS PB95126439), 1994.
Description:
Following intentional or accidental release, human exposure to biotechnology agents may occur. herefore, it is important to development, models to help identify any adverse health effects, either direct or indirect, that may be associated with the exposure. his study addresses colonization, competition, and translocation of biotechnology agents following intranasal challenge by sublethal doses of the environmental isolates Pseudomonas maltophilia, and P. aeruginosa. wo clinical isolates, P. aeruginosa strain PA01 and DG1 are included for comparison. 3H/HeJ mice received approximately 106 CFU of the individual strains i.n. he environmental isolates BC6 and AC869, as well as the clinical isolates, DG1 and PA01, were detectable in low numbers in all three intestinal sections 14 days following treatment. onversely, BC17 was cleared 7 days post-treatment. trains BC18 and BC16 wore detectable in 2 of the 3 sections at the end of the experimental time. he only overall treatment effect in the icrobiota monitored was a decrease in cecal lactose non-fermenting enteric bacilli in strain BC6 treated mice. ith the exception of BC6, all strains examined translocated to the MLN, spleen, and/or liver during the experimental time. he data obtained in this and previous studies suggests that exposure route in an important consideration when determining invasiveness of biotechnology agents.