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IN VITRO COMPETITION OF NATURAL 'AUTOGRAPHA CALIFORNICA' NUCLEAR POLYHEDROSIS VIRUS AND A RECOMBINANT EXPRESSING A POLYHEDRIN-BETA GALACTOSIDASE FUSION PROTEIN
Citation:
Kawanishi, C., Y. Huang, K. Bobseine, AND W. Setzer. IN VITRO COMPETITION OF NATURAL 'AUTOGRAPHA CALIFORNICA' NUCLEAR POLYHEDROSIS VIRUS AND A RECOMBINANT EXPRESSING A POLYHEDRIN-BETA GALACTOSIDASE FUSION PROTEIN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-88/259 (NTIS PB89129084), 1988.
Description:
The paper describes results of experiments conducted to investigate the kinetics of in vitro competition between natural progenitor Autographa californica (Acv E-2) and the recombinant Ac360-Bgal virus strains. The following conclusions can be drawn from the analysis: Selection pressure appeared to be fairly constant throughout the experiment; Selection was virtually unidirectional in favor of the natural strain regardless of the starting ratio, and consequently, the natural strain eventually predominated; The observed high frequency of mixed phenotype plaques appear to be influenced by some combinational phenomenon other than recombination at the DNA level; and The frequency of mixed phenotype plaques are also affected by passage. All evidence in the experiment points toward a disfavored recombinant genotype which appears not to be capable of competing effectively in vitro. The data present information that can be utilized as a basis for assessing the potential health effects of baculovirus expression vectors.