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DRUG RESISTANT ALLOREACTIVE T CELLS MAY CONTRIBUTE TO HUMAN GRAFT REJECTION
Citation:
Stanford, W., G. Strauss, AND O. Finn. DRUG RESISTANT ALLOREACTIVE T CELLS MAY CONTRIBUTE TO HUMAN GRAFT REJECTION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/175 (NTIS PB91115774), 1990.
Description:
The objective of our study was to determine whether resistance to immunosuppressive drugs by transplant recipient's T cells could contribute to continued graft rejection, in spite of immunosuppressive therapy. he T cell lines used in this series of experiments were originally established from T cells which have infiltrated kidney or liver grafts and initiated rejections in patients receiving immunosuppressive drugs, including the purine analogue azathioprine. e have used a proligeration assay and the Strauss-Albertini Test to analyze the T cell lines. oth assays used 6-thioguanie (6-TG), an amino derivative of AZ, as the selective agent to measure the resistance to AZ. lthough the frequency of 6-TG resistant variants in the majority of T cell lines closely resembled the frequency found in peripheral blood lymphocytes of controls, we identified four cell lines that demonstrated virtually complete resistance to the purine analog 6-TG in both assays. hese observations indicate that the more effective immunosuppressive treatment may be achieved by using a combination of drugs.
