You are here:
USE OF PERIFUSION TO EVALUATE HORMONAL RELEASE IN VITRO FROM RAT PITUITARY AND HYPOTHALAMIC TISSUE
Citation:
Goldman, J. AND R. Cooper. USE OF PERIFUSION TO EVALUATE HORMONAL RELEASE IN VITRO FROM RAT PITUITARY AND HYPOTHALAMIC TISSUE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/A-92/059 (NTIS PB92158534), 1992.
Description:
The use of in vitro procedures in reproductive toxicology has permitted a direct assessment of hormonal release from isolated tissue and a means by which to determine, potential sites of toxicant insult. he present chapter describes a perifusion procedure that can be used to evaluate stimulated or baseline hormonal secretion from pituitary and brain hypothalamic tissue. he implementation and utility of various combinations of tissue stimulation are presented along with discussion of the relative advantages and limitations of perifusion as an experimental approach.