You are here:
CLONING IN PSEUDOMONAS CEPACIA: EXPRESSION AND REGULATION OF THE PROTOCATECHUATE 3,4-DIOXYGENASE GENES
Citation:
Zylstra, G., R. Olsen, AND D. Ballou. CLONING IN PSEUDOMONAS CEPACIA: EXPRESSION AND REGULATION OF THE PROTOCATECHUATE 3,4-DIOXYGENASE GENES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/435 (NTIS PB90264771), 1989.
Description:
Genes for the a and B subunits of the enzyme protocatechuate 3,4-dioxygenase were cloned from the Pseudomonas cepacia DB01 chromosome on a 9.5 kilobase pair PstI fragment into the broad-host-range cloning vector pR023l7. he resultant clone was able to complement protocatechuate 3,4-dioxygenase mutations in ceoacia, P. cepacia, P. aeruginosa, and P. putida. xpression studies showed that the genes were constitutively expressed and subject to catabolite repression in the heterologous host. ince the cloned genes exhibited normal induction patterns when present in P. cepacia DB01 it was concluded that induction was subject to negative control.
URLs/Downloads:
CLONING IN PSEUDOMONAS CEPACIA: EXPRESSION AND REGULATION OF THE PROTOCATECHUATE 3,4-DIOXYGENASE GENES