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DNA ADDUCTS IN RAT LUNG, LIVER, AND PERIPHERAL BLOOD LYMPHOCYTES PRODUCED BY I.P. ADMINISTRATION OF BENZO(A)PYRENE METABOLITES AND DERIVATIVES
Citation:
Ross, J., G. Nelson, G. Erexson, A. Kligerman, K. Earley, R. Gupta, AND S. Nesnow. DNA ADDUCTS IN RAT LUNG, LIVER, AND PERIPHERAL BLOOD LYMPHOCYTES PRODUCED BY I.P. ADMINISTRATION OF BENZO(A)PYRENE METABOLITES AND DERIVATIVES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/306 (NTIS PB92124627), 1991.
Description:
DNA adducts produced in vivo in rat lung, liver, and peripheral blood lymphocytes following the i.p. administration of several synthetic benzo[a],pyrene (B[a]P) metabolites and ring-substituted derivatives have been analyzed by the nuclease P1 version of the 32P-postlabeling assay. hese include 1-, 2-, 3-, 4-, 5-, 6-, 7-, 8-, 9-, 10-, 11-, and 12-hydroxyB[a]P, (+)-B[a]P-trans-4,5-dihydrodiol, (+)-B[a]P-trans-7,8-dihydrodiol,(+)-B[a]P-trans-9,10-dihydrodiol, and B[a] P-7,8-dione. mong the monohydroxy derivatives, only 2-, 9-, and 12-hydroxyB[a]P produced detectable adducts. he only disubstituted derivative studied which produced adducts was the trans-7,8-dihydrodiol. he resulting DNA adducts were compared to those produced in each tissue by administration of B[a]P. -hydroxyB[a]P and B[a]P-trans-7,8-dihydrodiol each lead to the formation of major B[a]P adducts seen in lung and liver, respectively. one of the adducts derived from either 2-hydroxyB[a]P or 12-hydroxyB[a]P were observed following administration of B[a]P alone.