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IDENTIFICATION OF MUTAGENIC COMPONENTS IN WASTEWATER EFFLUENTS AND SLUDGES
Citation:
Tabor, M. AND J. Loper. IDENTIFICATION OF MUTAGENIC COMPONENTS IN WASTEWATER EFFLUENTS AND SLUDGES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/1-89/007 (NTIS PB90145731), 1989.
Description:
Both industrially-impacted and domestic municipal sewage treatment plant wastewaters and sludges have been studied to isolate the residue organics for the characterization of their mutagenic properties and for the isolation/identification of the mutagenic components. ethods were investigated to isolate the residue organics for mutagenic assessment via the Salmonella/microsomal activation, i.e., 59, assay. esidue organica were isolated from wastewaters by a modification of the USEPA/EMSL-LV interim protocol for the isolation of residue organics from drinking water for mutagenic assessment. esidue organics were isolated from sludges via three methods: the Soxhlet extraction method of Hites; the USEPA/EMSL-CIN Method 6245/6255; and a newly-developed method in this study that features the milling of the sludge with anhydrous sodium sulfate to a homogeneous powder followed by sequential extractions using a solvent series from non-polar to polar. he residue organics from wastewaters and sludges were fractionated by high performance liquid chromatography (HPLC) using a coupled bioassay/analytical fractionation approach. n general, mutagenic assessment of the residue organics from the industrially-impacted treatment plant wastewaters and sludges show both direct acting and 59 dependent TA98 mutagenic activity. o activity was observed for TAl00. nly l0% of the direct acting TA98 mutagenic activity observed for these sludge residue organics was found when the samples were bioassayed with TA98NR. sing a series of two preparative-scale HPLC separations, a subfraction containing approximately 70% of the TA98 mutagenic activity requiring metabolic activation in the effluent wastewater from the industrially-impacted plant was isolated. High resolution mass spectrometry analysis of this mutagenic subfraction showed three different chemical components. One was confidently identified as benzothiazole. he second component was tentatively identified as the ethylester of benzene sulfinic acid. he third component in this mutagenic subfraction did not give mass spectral results sufficient to suggest an identification. ased on bioassay results, the two identified components did not account for the mutagenic activity associated with this fraction.
