You are here:
RESULTS OF IPCS COLLABORATIVE STUDY ON COMPLEX MIXTURES
Citation:
Claxton, L., J. Creason, B. Leroux, E. Agurell, S. Bagley, D. Bryant, Y. Courtois, G. Douglas, C. Clare, S. Goto, P. Quillardet, D. Jagannath, K. Kataoka, G. Mohn, P. Nielsen, T. Ong, AND T. Pederson. RESULTS OF IPCS COLLABORATIVE STUDY ON COMPLEX MIXTURES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/058 (NTIS PB92150721), 1992.
Description:
The International Programme on Chemical Safety (IPCS) sponsored a collaborative study to examine the intra- and interlaboratory variation associated with the preparation and bioassay of complex chemical mixtures. The mixtures selected were National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM). wenty laboratories worldwide participated in the study. he participating laboratories extracted the organic portion of two particulate samples -- an air particulate sample and a diesel particulate sample -- and bioassayed the extracts. The laboratories imultaneously bioassayed a NIST-prepared extract of coal tar and two control ompounds (benzo(a)pyrene, and 1-nitropyrene). he bioassay method used was the Salmonella/mammalian microsome plate-incorporation test using strains TA98 and TA100. tudy design also allowed for a comparison of sonication and Soxhlet extraction techniques. he particulates samples were mutagenic in oth strains with and without activation in all twenty laboratories. he coal tar solution was negative for both strains when exogenous activation was not used but was mutagenic in both strains with exogenous activation. The benzo(a)pyrene and 1-nitropyrene were used as positive controls and gave results consistent with the literature. his paper provides a complete summary of the data collected during the collaborative study.