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COMPARISON OF MUTAGENICITY RESULTS FOR NINE COMPOUNDS EVALUATED AT THE HGPRT LOCUS IN THE STANDARD AND SUSPENSION CHO ASSAYS
Citation:
Moore, M., L. Parker, J. Houston, K. Harrington-Brock, AND K.L. Dearfield. COMPARISON OF MUTAGENICITY RESULTS FOR NINE COMPOUNDS EVALUATED AT THE HGPRT LOCUS IN THE STANDARD AND SUSPENSION CHO ASSAYS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/019 (NTIS PB91183475), 1991.
Description:
The Chinese hamster ovary (CHO) assay which measures newly induced mutations at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus has been widely used for mutagenesis testing. he insensitivity of the standard assay to some genotoxic agents has been speculated to be due to the relatively small number of cells used in the assay. n the present study, we have compared the standard monolayer assay with a suspension adapted assay which uses cell numbers comparable to that of the L5178Y mouse lymphoma assay. Nine compounds, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), 2-methoxy-6-chloro-9-[3-(ethyl-2-chloroethyl)-aminopropylamino]-acridine 2HCl (ICR 170), methyl acrylate, ethyl acrylate, tetraethylene glycol diacrylate, trimethylolpropane triacrylate, 2-ethylhexyl acrylate, and dicyclopentenyloxyethyl methacrylate were evaluated in the monolayer and suspension assays. oth assays gave the same overall positive/negative evaluation for the test compounds. here were some quantitative differences in the mutant frequency for the three compounds found to be mutagenic (EMS, MMS, and ICR 170). he acrylates (many of which appear to exert their genotoxic effect through a clastogenic mechanism) were negative in both test systems. he use of the suspension assay did not improve the ability of the locus to detect the genotoxicity of the acrylates.