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ISOFORMS OF APOLIPOPROTEIN A-I IN THE SERUM OF BROWN BULLHEADS (AMEIURUS NEBULOSUS) WITH LIVER CANCER
Citation:
Denslow, N., M. Chow, AND L. Folmar. ISOFORMS OF APOLIPOPROTEIN A-I IN THE SERUM OF BROWN BULLHEADS (AMEIURUS NEBULOSUS) WITH LIVER CANCER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/010, 1994.
Description:
1. Serum samples from 10 tumor-bearing (all fish with cholangiocellular carcinomas, some with both cholangio- and hepatocellular carcinomas) and 8 non-tumor-bearing brown bullheads were analyzed by 2D-PAGE. . Two isoforms of Apolipoproteins A-1 with a molecular weight of 28 kDA were identified. hose Isoforms had pls of 5.2 and 4.9. . Both isoforms were detected in fish with and with-out tumors. . There was a statistically significant increase in the amount of the more acidic protein (pi 4.9) in tumor bearing fish (46%) than in fish without tumors (28%). 5. There was a 50% (81% with conservative substitutions) homology of the N-terminal amino acid sequences between the brown bullhead and rainbow trout.