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EFFECT OF PARTICULATES ON OZONE DISINFECTION OF BACTERIA AND VIRUSES IN WATER
Citation:
Sproul, O., C. Buck, M. Emerson, D. Boyce, AND D. Walsh. EFFECT OF PARTICULATES ON OZONE DISINFECTION OF BACTERIA AND VIRUSES IN WATER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/2-79/089 (NTIS PB80116825), 1979.
Description:
The research was initiated in order to determine the effect of particulates on ozone disinfection of enteric bacteria and viruses adsorbed to or incorporated into particulate materials such as fecal material, HEp-2 cells, aluminum oxide floc and bentonite clay. Microorganisms used included fecal coliforms, poliovirus (Sabin Type 1), Coxsackievirus A9, porcine picorna-virus Type 3 (Strain ECPO-6), and f2 bacteriophage. The results indicate that the encasement or adsorption of enteric bacteria and viruses in fecal material, both human and porcine, and HEp-2 cells protects these microorganisms from a concentration of ozone and contact time that would normally inactivate the bacteria and viruses in an unadsorbed or free state. HEp-2 cells gave the greatest amount of protection for the cell-associated poliovirus and Coxsackievirus studied. It was necessary to maintain a concentration of 5.33 to 4.81 mg/l ozone to inactivate the cell-associated Coxsackievirus in 5 to 10 minutes. Hydrated aluminum oxide floc and bentonite clay afforded little or no protection to the Escherchia coli, poliovirus and Coxsackievirus adsorbed to these particles over that of the microorganisms in the free state. The f2 bacteriophage adsorbed to bentonite clay particles was inactivated at a slower rate than the freely suspended phage.