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CONSTRUCTION OF PLASMIDS FOR USE IN RISK ASSESSMENT RESEARCH
Citation:
Zylstra, G., S. Cuskey, AND R. Olsen. CONSTRUCTION OF PLASMIDS FOR USE IN RISK ASSESSMENT RESEARCH. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-88/216 (NTIS PB89118772), 1988.
Description:
The report describes a series of selftransmissible and nonselftransmissible (cloning vector) plasmids constructed to compare results from different laboratory tests and plasmid systems. Plasmids were designed to overcome problems of reproducibility, confusion due to use of different genetic structures with a wide range of capabilities, and background contamination when enumerating released organisms or recipients in gene transfer determinations. Inclusion of one or more such plasmids may alleviate some ambiguity in analysis of data from different environmental milieus.