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INFLUENCE OF SEDIMENT EXTRACT FRACTIONATION METHODS ON BIOASSAY RESULTS
Citation:
Ho, K.T., L.J. Mills, C. Mueller, S. Anderson, AND R. Malcolm. INFLUENCE OF SEDIMENT EXTRACT FRACTIONATION METHODS ON BIOASSAY RESULTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/481 (NTIS PB95136859), 1994.
Description:
Four bioassays [Microtax(tm), Mutatox(tm), sister chromatid exchange (SCE), and metabolic cooperation] were used to analyze marine sediment extracts fractionated by two different methods: silica gel column chromatography and acid-base fractionation. esults indicated that a sediment extract fractionated with different methods can lead to different conclusions about the same sediment.This research also further evaluated the new, mutagenic, bacterial bioassay Mutatox. utatox generally correlated with SCE and the Salmonella typhimurium assays. his rapid, operationally simple bioassay has potential as a screening bioassay to detect genotoxic agents. 01994 by John Wiley & Sons, Inc.
