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GENE ENGINEERING IN YEAST FOR BIODEGRADATION: IMMUNOLOGICAL CROSS-REACTIVITY AMONG CYTOCHROME P-450 SYSTEM PROTEINS OF SACCHAROMYCES CEREVISIAE AND CANDIDA TROPICALIS
Citation:
Loper, J., C. Chen, AND C. Dey. GENE ENGINEERING IN YEAST FOR BIODEGRADATION: IMMUNOLOGICAL CROSS-REACTIVITY AMONG CYTOCHROME P-450 SYSTEM PROTEINS OF SACCHAROMYCES CEREVISIAE AND CANDIDA TROPICALIS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/307 (NTIS PB93229292), 1985.
Description:
Yeasts are eukaryotic microorganisms whose cytochrome P-450 monoxygenase systems may be amenable to genetic engineering for the hydroxylation and detoxication of polychlorinated aromatic hydrocarbons. e are examining the molecular genetic properties of strains of bakers yeast, Saccharomyces cerevisiae, and an n-alkane utilizing yeast, Candida tropicalis ATCC750. tandard methods were used to purify cytochrome P-450 and NADPH-cytochrome c (P-450) reductase proteins f rom cells cultured by semi-anaerobic glucose fermentation (S. cerevisiae, C. tropicalis) and by growth on tetradecane (C. tropicalis). olyvalent antisera prepared in rabbits to some of these proteins were used in tests of immunological relatedness among the purified proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and nitrocellulose filter immunoblots. he results provide evidence for gene relationships which should prove useful in gene isolation and subsequent engineering of P-450 enzyme systems in yeast.
