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INTERFACE OF A REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPH WITH A DIFFUSE REFLECTANCE FOURIER TRANSFORM INFRARED SPECTROMETER
Citation:
Conroy, C., P. Griffiths, P. Duff, AND L. Azarraga. INTERFACE OF A REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPH WITH A DIFFUSE REFLECTANCE FOURIER TRANSFORM INFRARED SPECTROMETER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-84/228 (NTIS PB85160422), 1984.
Description:
An approach to the interface of a reverse-phase high-performance liquid chromatograph and a Fourier transform infrared spectrometer has been developed in which the solutes eluting from the column are continuously extracted into dichloromethane. The application of both flow cell and solvent elimination techniques was explored. Coextracted water and methanol into the CH2Cl2 precluded the use of flow cells. In the solvent elimination interface, the solutes are first extracted into dichloromethane and subsequently concentrated by differential evaporation. The solutes are then deposited on gently heated KC1 powder, and their diffuse reflectance spectra are measured. The interface operates in a continuous fashion without the need for an auxiliary detector. Spectra can be measured at submicrogram sensitivity using this interface. (Copyright (c) 1984 American Chemical Society.)