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DNA ADDUCT RESEARCH WITH CAPILLARY ELECTROPHORESIS
Citation:
Norwood, C., E. Jackim, AND S. Cheer. DNA ADDUCT RESEARCH WITH CAPILLARY ELECTROPHORESIS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/487 (NTIS PB94130242), 1993.
Description:
DNA's central importance in all biological systems dictates a wide variety of DNA-related research. or much of this research, the utilization of capillary electrophoresis (CE) can be of significant advantage. pen-tube CE yields excellent separations of DNA components, which can be monitored in a nearly "real-time" manner by uv absorbance detection. imilar CE techniques enable the monitoring and optimization of enzymatic reactions. uring DNA hydrolysis, both the reactants and the products can be simultaneously observed. NA adducts (covalent additions of compounds to the DNA components) can similarly be analyzed by CE with uv detection. any adduct nucleotides elute in a region of the electropherogram free from interferences due to the non-adducted components. mproved limits of detection are achieved when sample stacking techniques are utilized. mproved separations can result when the CE analysis involves micellar electrokinetic capillary chromatography.