Citation:

Sanders, M., L. Martin, W. Nelson, D. Phelps, AND W. Welsh. RELATIONSHIPS BETWEEN ACCUMULATION OF A 60KDA STRESS PROTEIN AND SCOPE-FOR-GROWTH IN MYTILIS EDULIS EXPOSED TO A RANGE OF COPPER CONCENTRATIONS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/315 (NTIS PB93229375), 1991.

Description:

Blue mussels, Mytilus edulis, were exposed in the laboratory for 7 days to a range of Cu concentrations (O to 100 ug/liter) to test the feasibility of using the accumulation of a 60 kDa stress protein, also referred to as heat shock protein 60 (hsp60) as a molecular indicator of contaminant exposure and ecological status. t the end of the experiment, scope-for-growth (SFG) measurements were completed to assess whole organism physiological condition. n addition, antibodies raised against hsp60 were employed as probes for Western blot analysis and immunobinding assays of mantle tissue. hysiological measurements revealed a significant (p < 0-05) decrease in the SFG index for organisms at 32 and 100 ug/liter Cu. estern blot analysis indicated that the abundance of hsp60 as greater in tissues exposed to elevated Cu concentrations than in controls. uantitation of hp60 accumulation as determined by a dot immunobinding assay demonstrated a significant correlation between hsp60 accumulation and nominal Cu concentration in the seawater. ignificant (p < 0-1) increases in accumulation of hsp60 over controls was detected at 3-2 ug/liter Cu and higher. u concentrations which were substantially lower than that at which a significant decrease in SFG was observed. hese data suggest that hsp60 accumulation may hold promise as a molecular indicator of contaminant exposure and an early warning of adverse biological effects.

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